Effect of BAP and 2,4-D on callus induction of Jatropha curcas in vitro

Abstract. Andaryani S, Samanhudi, Yunus A. 2019. Effect of BAP and 2,4-D on callus induction Jatropha curcas in vitro. Cell Biol Dev 3: 56-65. The aim this study was to obtain appropriate concentrations Benzyl Amino Purine (BAP) 2,4-dichlorophenoxyacetic acid (2,4-D) increase vitro using shoot explants L. research conducted at the Laboratory Plant Physiology Biotechnology, Faculty Agriculture, Universitas Sebelas Maret, Surakarta, Central Java, Indonesia, from July August 2010. used a completely randomized design (CRD) with two treatment factors three replications. first factor is level concentration, namely: 0.5 ppm, 1 1.5 2 ppm. second concentration 2,4-D, 0 0.25 0.75 Observation variables included emergence time, color, texture, root number roots, shoots, leaf leaves, fresh weight. Qualitative data are presented descriptively. Quantitative includes weight data, which analyzed for variance based 5% F test continued DMRT level. results showed that combination ppm fastest inducing callus, 5.67 Days After Planting (DAP). All resulting calluses have crumb texture. average color all combinations yellowish-green. Only without could produce roots 9 DAP. 6 obtained 11 resulted largest (2.56 g).